Aeromonas hydrophila: Antimicrobial Susceptibility and Histopathology of Isolates from Diseased Catfish, Clarias gariepinus (Burchell)

Lesions from skin, fin and gill, were inoculated onto Trypticase Soy Agar (TSA) (Merk, Germany) and incubated aerobically at 28C for 24 h. Then internal samples were acquired alike aseptically from the kidney, liver, spleen and the infected muscles. The plates were examined for bacterial growth. Dominant colonies were selected, re-streaked on Trypticase Soy Agar (TSA). Cultures were placed in 20% glycerol and supplemented in Trypticase Soy Broth (TSB) for storage at -80C. Dominant colonies were selected for bacterial isolation to establish the optimal number of bacterial cells, and undergoing thorough purification procedure until pure colonies were established to be sure that dominant colonies were not contaminated.