Isoelectric focusing on immobilized pH gradients dissociates most complexes between fatty acids and serum albumin from whole plasma. Most albumin thus migrates to a pI of 5.8, very close to the theoretical value of 6.0 as calculated with the Linderstrøm‐Lang equation. The phenomenon was elucidated through saturation experiments and time‐course monitoring of the protein pattern upon focusing.
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Gianazza et al. (1984) studied this question.
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