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We applied laser microtomography, a direct optical section technique using a confocal laser scanning microscope, to the mapping of subcellular three-dimensional morphology. A novel model of laser microscope in combination with a fine focus control device and a digital image processor enables us to readily reconstruct high resolution, extended-focus, three-dimensional images at any viewing angle. This system facilitates recognition of the in situ morphology of such subcellular components as nuclear chromosomes or cytoskeletons.
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Minamikawa et al. (1991) studied this question.
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