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Five translocation heterozygotes were induced by using gamma rays in Pisum sativum. The induced translocations showed a ring or chain of 4 chromosomes and 5 bivalents in most of the PMCs at diakinesis/metaphase I. Translocation heterozygotes predominantly showed an adjacent type of orientation as compared to alternate orientations of the centromeres which is reflected in the pollen stainability and pollen fertility of the plants. The translocation heterozygotes established could be used for gene mapping and the production of trisomics They would have altered karyotypes useful for other experimental studies on Pisum sativum.
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Verma et al. (2012) studied this question.
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