Phytopharmacological evaluation of combined extract of Trigonella foenum-graceum and Holarrhena pubescens for the management of alloxan induced type-2 diabetes in rats

Objectives: To evaluate the antioxidant, antidiabetic effect of the polyherbal formulation of methanolic extract of Trigonella foenum-graceum Holarrhena pubescens (seed) and(stem) on alloxan induced type-2 diabetes in rats.Materials and Method: Rats were divided into five groups (n=6).Group-I (Normal control) received normal saline.Group-II (Negative control) received 120 mg/kg of Alloxan, treated subsequently with normal saline.Group-III and IV (Test group Test-1&2) received 250, 500 mg/kg bw of TFG+HP methanolic extract.Group-V (Standard) received Glibenclamide (3mg/kg p.o).The qualitative analysis of various phytochemical constituents and quantitative analysis were determined.Theantidiabetic effects studied using in vivo glucose uptake in an isolated alloxan-induced diabetic rat model.The anti-oxidant activity of extracts of and was in vitroTrigonella foenum-graecum Holarrhena pubescens evaluated by DPPH radical scavenging activity.Their phytochemical analysis revealed variable Results: phytoconstituents in the methanolic extracts.In vitro antioxidant activity was measured using the one, DPPH assay, with methanolic extracts from and showing IC values of 37.05 Trigonella foenum-graecum Holarrhena pubescens 50 and 32.32, respectively, close to the standard (Ascorbic acid).The test samples showed a significant (P<0.05)reduction in blood glucose levels compared to the alloxan-treated group.The body weights for the normal control, alloxantreated, and polyherbal formulation-treated groups (at doses of 250 mg and 500 mg) were recorded.Conclusion: The results obtained in present study clearly demonstrate that the methanolic extracts from Trigonella foenum-graecum seeds and stems exhibited significant antioxidant activity and moderate antidiabetic activity, suggesting H. pubescens the need for further exploration in animal models and the isolation of active constituents.