A General Approach for the Identification of Site‐Specific RNA Binders by 19 F NMR Spectroscopy: Proof of Concept

Bound to succeed: Noncovalent binding of ligands to RNA can be analyzed easily by 19F NMR spectroscopy through site-specific fluorine-labeling of the target RNA (see picture; B=adenine, cytosine, guanine, uracil). The proof of concept is provided by RNA aptamer–ligand interactions. The speed and the robustness of this simple approach present major advantages over methods relying on 1H NMR spectroscopy.