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Cowpea (Vigna unguiculata L.) leaf discs incubated in the dark in CaCl2 and benzyladenine maintained higher levels of chlorophyll and protein than controls. The CaCl2 or benzyladenine treatments reduced lipoxygenase activity, and the effect of these compounds in combination was additive. HPLC analysis of the product profile of lipoxygenase activity with arachidonic acid as a substrate showed a single peak comigrating with standard 15‐hydroperoxyeicosatetraenoic acid. There appears to be a strong temporal correlation between the CaCl2+ benzyladenine delay of senescence and lipoxygenase activity.
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P. M. Swamy (1992) studied this question.
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