Präparative gewinnung hochgereinigter lysosomenenzyme aus rattenlebern

By means of a relatively simple procedure lysosomal enzymes from rat livers with 20% yield and a 50‐fold enrichment were prepared. Density gradient centrifugation or tissue‐preloading with triton VR 1339 is not necessary. Up to several liters of 50% liver homogenate may be used. Intact lysosomes are not obtained, because the crucial step in the tissue fractionation scheme is the selective release of lysosomal enzymes from a crude lysosome pellet by water extraction. The presence of neutral endopeptidases with high specific activities in lysosomes (estimated using azocasein and other proteins as substrates) is demonstrated.