Key result
Transcription of the Trypanosoma brucei spliced leader RNA gene is mediated by an alpha-amanitin/tagetitoxin resistant complex and depends only on upstream regulatory elements.
The study demonstrates that T. brucei SL RNA gene transcription relies solely on upstream regulatory elements, distinguishing its promoter architecture from that of nematodes.
Distinguishes T. brucei SL RNA promoter architecture from nematodes; leaves open selective targeting for future antiparasitic research.
The spliced leader (SL) RNA plays a key role in mRNA maturation in trypanosomatid protozoa by providing the SL sequence, which is joined to the 5' end of every mRNA. As a first step towards a better understanding of the biogenesis and function of the SL RNA, we expressed a tagged SL RNA gene in a cell-free system of procyclic Trypanosoma brucei cells. Transcription initiates at + 1 can be detected as early as 1 min after addition of extract. Transcription of the SL RNA gene in vitro, as well as in permeable cells, is mediated by an alpha-amanitin/tagetitoxin resistant complex, suggesting a promoter that is intermediate between a classical RNA polymerase II and RNA polymerase III promoter. An analysis of the promoter architecture of the SL RNA gene revealed that regulatory elements are located upstream of the coding region and that the SL sequence, in contrast to the nematode SL sequence, is not required for T. brucei SL RNA gene transcription.
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Günzl et al. (1997) studied Trypanosoma brucei. Expression of tagged SL RNA gene in a cell-free system was evaluated on Transcription of the SL RNA gene. Transcription of the Trypanosoma brucei spliced leader RNA gene is mediated by an alpha-amanitin/tagetitoxin resistant complex and depends only on upstream regulatory elements.
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