Key result
Mutations Ser408Glu, Pro409Lys, Tyr477Ala, and Leu485Lys in the Ad5 fiber knob effectively abolished high-affinity binding to the coxsackievirus and adenovirus receptor (CAR).
Population
Preclinical models including recombinant Ad5 fiber knob proteins, soluble extracellular domains of CAR, and…
Comparison
Site-directed mutagenesis of Ad5 fiber knob vs Wild-type Ad5 fiber knob
Design
Preclinical
Authors
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May enable CAR-independent adenoviral vectors for gene therapy; leaves open in vivo efficacy and specificity.
Identification of critical contact residues on the Ad5 fiber knob for CAR binding provides structural insights for developing novel adenoviral vectors for targeted gene therapy.
Kirby et al. (2000) studied Adenovirus infection (basic science). Site-directed mutagenesis of Ad5 fiber knob vs. Wild-type Ad5 fiber knob was evaluated on Binding affinity to CAR (Kd). Mutations Ser408Glu, Pro409Lys, Tyr477Ala, and Leu485Lys in the Ad5 fiber knob effectively abolished high-affinity binding to the coxsackievirus and adenovirus receptor (CAR).
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