The germinal crescent of head-fold and one-somite-stage chicken embryos was irradiated with multiple pulses of a microbeam ruby laser. Primordial germ cells were not detected in the gonad primordium of six laser-irradiated 5-day embryos; ten laser-irradiated embryos had varying numbers of primordial germ cells. Ten control embryos had gonad primordia populated with many primordial germ cells.
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Mims et al. (1971) studied this question.
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