Comparison of Chemical and Antimicrobial Studies of Egyptian Mandarin Leaves and Green Branches Volatile Oil

Aims : In the present study we compared the chemical composition and the antimicrobial activity of the essential oils isolated from green branches and leaves of mandarin. Study Design : Extraction of essential oils from mandarin green branches and leaves by hydrodistillation Place and Duration of Study : leaves and green branches were collected from Benha City, Qualyobi a, Egypt in December 2012. Methodology: The essential oils (EOs) isolated from mandarin ( Citrus reticulata ) were analyzed by GC and GC/MS. The antimicrobial activity was assessed using agar well diffusion method. Results : Sixteen compounds were identified from the oil of branches which represented about 92% of the total detected constituents. The major components of the oil were dimethyl anthranilate (34.7%), γ-terpinene (33.6%) and limonene (11.2%). Alpha pinene and sabinene were present in considerable amounts (both at 2.8%). Other components were present in amounts less than 2%. From the heavier of layer mandarin leaves oil, thirteen compounds accounting for 95.4% of the oil were identified. Dimethyl anthranilate (65.3%) was the major component, followed by γ-terpinene