A 42-gene thromboinflammation platelet signature (TIPS) predicted future cardiovascular events in patients who underwent lower extremity revascularization (adjHR 1.55, P=0.006).
Cohort (n=149)
Does a platelet transcriptomic signature of thromboinflammation predict cardiovascular risk?
A novel 42-gene platelet RNA signature (TIPS) captures thromboinflammation, predicts future cardiovascular events, and is modifiable by ticagrelor.
Hazard Ratio: 1.55
p-value: p=0.006
BACKGROUNDPlatelets are increasingly recognized as active participants in immune signaling and systemic inflammation. Upon activation, platelets form monocyte platelet aggregates (MPA) representing the crossroads of thrombosis and inflammation. We hypothesized that platelet transcriptomics could capture this thromboinflammatory axis and identify individuals at elevated cardiovascular risk.METHODS: MPA levels, defined as CD14+CD61+ cells, were measured using flow cytometry at 2 time points, 4 weeks apart, in healthy individualsPlatelets were isolated and sequenced. Individuals were categorized as MPAhi or MPAlo based on consistently high or low MPA levels across time points.RESULTSAmong 149 participants (median age 52 years, 57% female, 50% non-White), MPAhi individuals exhibited increased expression of platelet activation markers P-selectin (P < 0.001), PAC-1 (P = 0.021), and CD40L (P < 0.001) and enriched immune signaling pathways. Informed by MPA levels and derived from the platelet transcriptome, we developed a 42-gene thromboinflammation platelet signature (TIPS), which correlated with MPA levels in multiple cohorts and was reproducible over time. TIPS was elevated in patients with COVID-19 (P = 0.0002) and myocardial infarction (Padj = 0.008), and as in predicted future cardiovascular events in patients who underwent lower extremity revascularization after a median follow-up of 18 months (adjusted for age, sex, race, and ethnicity adjHR 1.55, P = 0.006). Notably, TIPS was modifiable by ticagrelor (P = 0.002) but not aspirin.CONCLUSIONThese findings establish MPA as a biomarker of thromboinflammation and introduce TIPS, a platelet RNA signature, that captures thromboinflammation and provides a promising tool for cardiovascular risk stratification and a potential therapeutic target.TRIAL REGISTRATIONNCT04369664FUNDINGNIH R35HL144993, NIH R01HL139909, and AHA 16SFRN2873002 to JSB, DFG Walter-Benjamin-Programme 537070747 to AB.
A 2025 study conducted a cohort in Cardiovascular risk (n=149). Thromboinflammation platelet signature (TIPS) vs. Lower TIPS expression was evaluated on Future cardiovascular events (adjHR 1.55, p=0.006). A 42-gene thromboinflammation platelet signature (TIPS) predicted future cardiovascular events in patients who underwent lower extremity revascularization (adjHR 1.55, P=0.006).