Key result
C-terminal truncation variants of YsxC were unable to bind to the 50S ribosome and rRNAs, indicating the basic C-terminal region is essential for binding.
The basic C-terminal region of YsxC is essential for its binding to the 50S ribosomal subunit.
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C-terminal YsxC region is essential for ribosomal binding; extends molecular insights into bacterial translation but remains hypothesis-generating.
YsxC is an essential P-loop GTPase that interacts with the 50S subunit of the ribosome. The putative implication in ribosome binding of two basic clusters of YsxC, a conserved positively charged patch including R31, R116, H117 and K146 lying adjacent to the nucleotide-binding site, and the C-terminal alpha helix, was investigated. C-terminal truncation variants of YsxC were unable to bind to both ribosome and rRNAs, whereas mutations in the other cluster did not affect YsxC binding. Our results indicate that the basic C-terminal region of YsxC is required for its binding to the 50S ribosomal subunit.
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Wicker‐Planquart et al. (2015) studied this question. C-terminal truncation variants of YsxC vs. Mutations in the other basic cluster (R31, R116, H117, K146) was evaluated on Binding to 50S ribosome and rRNAs. C-terminal truncation variants of YsxC were unable to bind to the 50S ribosome and rRNAs, indicating the basic C-terminal region is essential for binding.
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