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A method for the extraction and assay of male and female hormones from normal urines has been described. 2. This procedure consists in 2-hour acid hydrolysis of the urine, extraction with ten times the volume of benzene using the extractor described, and separation into male and female fractions by alkali. 3. The assay is conducted with standards in parallel and results expressed in international units. 4. An alternative procedure for male hormone alone using an adsorption process is likewise described.
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Gallagher et al. (1935) studied this question.
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