The quantitative sterol requirements were studied in C. briggsae, C. elegans (Be), C. elegans (Br), and T. aceti. It was shown that all four nematodes had similar minimal sterol requirements (0.1-2.0 μg/ml) and toxicity appeared in T. aceti at 50 μg/ml. Cholesterol and five precursors were tested for population growth. We found that acetic acid, DLmevalonic acid lactone, and farnesol did not support population growth; while squalene, lanosterol, and cholesterol supported significant population growth in all four nematodes. Our results suggest that the major metabolic block in the pathway of sterol biosynthesis occurs between the step of farnesol and squalene.
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Stokstad et al. (1977) studied this question.
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