Effect of Nephropathy Prescription I on the Expression of Angptl3 and Podocyte-Associated Protein in Mice with Adriamycin-Induced Nephropathy

Figures

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  Figure 1. Changes in body weight (g) in different groups: control group (C), adriamycin group (M), adriamycin + Nephropathy Prescription I group (M + Z), adriamycin + prednisone acetate group (M + S), and adriamycin + Nephropathy Prescription I + prednisone acetate group (M + Z + S).

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  Figure 2. Changes of urine albumin/creatine ratio in different groups (mg/mg): control group (C), adriamycin group (M), adriamycin + Nephropathy Prescription I group (M + Z), adriamycin + prednisone acetate group (M + S), and adriamycin + Nephropathy Prescription I + prednisone acetate group (M + Z + S).

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  Figure 3. Changes in serum albumin in different groups (g/L): control group (C), adriamycin group (M), adriamycin + Nephropathy Prescription I group (M + Z), adriamycin + prednisone acetate group (M + S), and adriamycin + Nephropathy Prescription I + prednisone acetate group (M + Z + S).

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  Figure 4. Changes in total cholesterol and triglyceride levels in different groups (mmol/L): control group (C), adriamycin group (M), adriamycin + Nephropathy Prescription I group (M + Z), adriamycin + prednisone acetate group (M + S), and adriamycin + Nephropathy Prescription I + prednisone acetate group (M + Z + S).

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  Figure 5. Pathological changes of glomerulus under optical microscopy (HE × 400, PAS × 400) in different groups: control group (C), adriamycin group (M), adriamycin + Nephropathy Prescription I group (M + Z), adriamycin + prednisone acetate group (M + S), and adriamycin + Nephropathy Prescription I + prednisone acetate group (M + Z + S).

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  Figure 6. Electron microscopy images of morphological changes in foot processes in mice with nephropathy (× 7000) of different groups: control group (C), adriamycin group (M), adriamycin + Nephropathy Prescription I group (M + Z), adriamycin + prednisone acetate group (M + S), and adriamycin + Nephropathy Prescription I + prednisone acetate group (M + Z + S). There was no significant change in the structure of glomerular podocytes in the C group; the glomerular basement membrane in the M group was thickened, and foot processes were diffusely fused or disappeared; foot processes in the (M + Z) group were partially fused; foot processes were clearer in the (M + Z + S) group.

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  Figure 7. mRNA levels of nephrin, podocin, and Angptl3 in different groups. ∗M vs. C group (P < 0.05); &M + Z or M + S vs. M group (P < 0.05); #M + Z + S vs. M group (P < 0.01).

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  Figure 8. Protein levels of nephrin, podocin, and Angptl3 in different groups: (a) control (C); (b) model group (M); (c) traditional Chinese medicine (TCM) group (M + Z); (d) (M + S); and (e) (M + Z + S). ∗M vs. C group (P < 0.05); &M + Z or M + S vs. M group (P < 0.05); #M + Z + S vs. M group (P < 0.01).