Key result
Mutating the nucleotide sequence surrounding the first methionine start site to a Kozak consensus sequence produced Kv3.3 currents with fast and voltage-dependent inactivation in CHO and HEK cells.
Population
Chinese hamster ovary and human embryonic kidney cells expressing AptKv3.3
Comparison
Mutation of the nucleotide sequence surrounding… vs Wild-type AptKv3.3 sequence
Design
Preclinical
Authors
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Optimized Kozak sequence confers fast inactivation to Kv3.3 in vitro; leaves open relevance to native neuronal A-type currents.
Under appropriate conditions with a strong Kozak consensus sequence, Kv3.3 channels can exhibit fast and reliable inactivation approaching that of 'A'-type K+ currents.
Fernandez et al. (2003) studied this question. Mutation of the nucleotide sequence surrounding the first methionine start site to a Kozak consensus sequence vs. Wild-type AptKv3.3 expression was evaluated on Rate and voltage dependence of inactivation. Mutating the nucleotide sequence surrounding the first methionine start site to a Kozak consensus sequence produced Kv3.3 currents with fast and voltage-dependent inactivation in CHO and HEK cells.
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