Key result
Among 31 herbal formulae tested, Chokyungjongok-tang, Oncheong-eum, and Cheongsangbangpung-tang strongly inhibited hERG channel activity with IC50 values of 67.67, 141.2, and 296.3 μg/mL, respectively.
Why the study?
Drug-induced blockade of the hERG potassium ion channel causes acquired long QT syndrome, leading to potentially fatal cardiac arrhythmias, prompting evaluation to establish safety evidence for herbal formulae.
Do 31 herbal formulae inhibit hERG channel activity in HEK 293 cells?
Do 31 herbal formulae inhibit hERG channel activity in HEK 293 cells?
Three specific herbal formulae strongly inhibit hERG channel activity in vitro, suggesting a potential risk for drug-induced long QT syndrome and cardiac arrhythmias.
May warrant arrhythmia risk caution with these formulae; leaves open clinical relevance pending further studies.
Objectives: Drug-induced blockade of the human ether-à-go-go related gene (hERG) potassium ion channel causes acquired long QT syndrome, which is known to cause cardiac arrhythmias and be fatal. To establish safety evidence of herbal formulae, we evaluated the effects of 31 herbal formulae on hERG channel activity.Methods: The current through hERG channel was measured by changing the membrane voltage before and after treatment with 31 herbal formulae in HEK 293 cell overexpressing hERG channel using a whole-cell patch clamp system. The current-voltage curves and the activity curves were fitted, and the hERG activity and 50% inhibitory concentration (IC50) according to each herbal formula were calculated.Results: Chokyungjongok-tang, Oncheong-eum, and Cheongsangbangpung-tang strongly inhibited the hERG activity, with IC50 values of 67.67, 141.2, and 296.3 μg/mL, respectively. Yeonkyopaedok-san, Eunkyo-san, Ukgan-san gajinphibanha, Daegunjoong-tang (except Oryzae gluten), Insamyangyoung-tang, Banhahubak-tang, SokyungHwalhyul-tang, Jodeung-san, Hyeonggaeyeongyo-tang, and Bangkeehwangkee-tang weakly inhibited hERG activity, with IC50 values ranging from 400 to 1000 μg/mL. The other 18 herbal formulae showed very weak hERG activity inhibition of less than 50% at the highest concentration (1000 μg/mL).Conclusion: This study provided safety information on cardiotoxicity by cardiac arrhythmia risk assessment of herbal formulae, and is expected to be a reference data for predicting the safety and risk of herbal formulae.
No takes yet. Share an insight, caveat, or question.
Ha et al. (2022) studied Cardiotoxicity. 31 herbal formulae vs. Quinidine (positive control) and external buffer was evaluated on hERG channel activity inhibition (IC50). Among 31 herbal formulae tested, Chokyungjongok-tang, Oncheong-eum, and Cheongsangbangpung-tang strongly inhibited hERG channel activity with IC50 values of 67.67, 141.2, and 296.3 μg/mL, respectively.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: