Summary Immunoelectrophoresis of lymph from the hind paws of rabbits before and after thermal injuries of differing intensities showed that, when an antiserum to rabbit serum was used all the proteins detected in the circulating blood serum were also present in the lymph. This suggests that all the plasma proteins take part in the increased movement of proteins through the extra‐ cellular fluid phase of an injured tissue. However, when an antiserum to rabbit lymph collected from thermally Injured paws was employed, Immunoelectrophoresis showed an extra protein in the lymph from injured paws which was not present in normal serum or lymph, or in the serum after injury. This extra protein migrated as a β2‐ or fast γ‐globulin It was found after severe injuries at 70 and 80°, but not after the less severe injuries at 55 to 60°. Its appearance was transient, lasting for only 1 to 2 hr. after injury. Further experiments showed that this protein was not Cx‐reactive protein and that it was not produced by heating the normal extracellular fluid proteins. Preliminary experiments to test its antigen city within the species have proved negative. The levels of the enzymes lactic dehydrogenase glutamie oxalacetic transaminase and glutamic pyretic transaminase were also increased in the lymph after injury. but experiments by Immunoelectrophoresis suggest that the extra protein is probably not identical with these enzymes.
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Roberts et al. (1969) studied this question.