It is shown that pressure affects the extent of preferential sorption of mixed eluent components on the surface of GPC column packing. In some systems, this may result in base line perturbations and possibly also in changes of size, shape and position of sample peaks when pressure is changed within column either due to variation in the flow rate or as a result of partial blocking of column end fittings and connecting capillaries. The described phenomenon could lead to erroneus GPC results. On the other hand, it may help to explain some unexpected and artificial results obtained in GPC separations with mixed eluents.
No takes yet. Share an insight, caveat, or question.
Macko et al. (1986) studied this question.
Synapse has enriched 5 related papers. Consider them for comparative context:
More from Journal of Liquid Chromatography