Key result
An automated method for quantitation of levosimendan in human plasma demonstrated a between-day variation of 1.7-2.7% and a limit of quantitation of 5 ng/mL.
This study describes a rapid and precise automated analytical method for quantifying the inotrope levosimendan in human plasma.
May enable levosimendan monitoring in research; leaves open clinical validation before routine use.
A fully automated method for quantitation of levosimendan, (R)-(-)-[[4-(1,4,5,6-tetrahydro-4-methyl-6-oxo-3-pyridazinyl) phenyl]hydrazono]propanedinitrile, in human plasma is described. The method involves on-line dialysis of the samples, trace enrichment of the dialysates, and reversed-phase high-performance liquid chromatography with UV detection at 380 nm. An internal standard was used to compensate for variations in the dialysis rate caused by temperature fluctuations. The precision and accuracy of the method were good. The between-day variation (RSD) was 2.7% at a plasma concentration of 15 ng/mL and 1.7% at 450 ng/mL. The limit of quantitation was 5 ng/mL with an RSD of 4.0%. The completion time of the assay was 19 min.
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Karlsson et al. (1997) studied this question. Automated analysis of levosimendan by on-line dialysis and liquid chromatography was evaluated on Precision, accuracy, and limit of quantitation. An automated method for quantitation of levosimendan in human plasma demonstrated a between-day variation of 1.7-2.7% and a limit of quantitation of 5 ng/mL.
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