Genetic Similarity of Geographic Caulerpa(Chlorophyta)Populations in Taiwan Island Revealed by Random Amplified Polymorphic DNA

In 1996,anguillcolosis were observed causing a mass mortality of the intensive Americaneel,Anguilla rostrata,aquaculture facility in central Taiwan.Examining the matureadult worms from the lumen of swim bladder in infected eels,the nematode was identifiedas Anguillicola crassus based on the morphology of esophagus of adult worm and thenumber of circumoral teeth(n=25).Merthiolate-iodine-formalin(MIF)stain method,agood stain preservative for most kinds and stages of parasites found in feces,is used toobserved the eels infected by Anguillicola in this examination.The specificity and predictivevalue positive of MIF were both 100%,and the sensitivity and predictive value negativewere 56% and 36% respectively.The method of MIF is cheap and convenient to bepromoted in examining of anguillicolosis in aquaculture facility.