Preparation of Solanesol from a Tobacco Leaf Extract Using High Speed Countercurrent Chromatography

Separation of solanesol from the tobacco leaves extract was performed by high speed countercurrent chromatography using a solvent system composed of petroleum ether‐ethanol‐methanol (200∶1∶100, v/v). In each separation, one gram of the tobacco leaves extract was injected to the multilayer coil separation column to yield 121 mg of solanesol with a purity of 90.7%. The obtained solanesol was identified by ESI‐MS, 1H, and 13C‐NMR.