A micro enzyme-sensor using uricase (EC 1.7.3.3) was developed for measuring uric acid. For this, a sheet of porous composite was made from acetylene black and Teflon emulsion and was soaked in Zonyl FSN fluoro-carbon surfactant and Nafion. The 50 μ m platinum micro-electrode was etched in hot aqua regia to create a cavity at its tip and this cavity was packed with porous composite material by pushing the tip into small lumps of the material. The redox mediator [Os(bpy)3 (PF6)2] was monitored by cyclic voltammetry. The porous micro-sensor was dipped overnight in Tris-hydrochloric acid buffer of pH 8.0 containing enzyme for adsorption of uricase at 4°C. The effects of pH, and temperature on measurements of uric acid with the sensor were examined, the calibration curve for measurement of uric acid under optimum conditions was determined and the effect of concomitant compounds were investigated. Under optimum conditions, the response of the micro-sensor was linear in the concentration range of 0.101 - 1.93mM uric acid. The uric acid concentrations in commercial preparations and human serum were also determined with this sensor.
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Motonaka et al. (1994) studied this question.
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