Enzymic Preparation ofL-β-Lysine

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L-β-Lysine is prepared from L-lysine by the action of an enzyme, L-lysine-2, 3-aminomutase, present in extracts of lysine-fermenting Clostridia. 1The basic amino acids in the reaction mixture are adsorbed on a cation exchange resin, and β-lysine is separated from residual lysine by differential elution at pH 3.0. The β-lysine is desalted by adsorbing it on a cation exchange resin, washing the resin with water and eluting the 8-lysine with ammonia. Ammonia is removed and the β-lysine is converted to the sulfate and crystallized from aqueous methanol. The yield is 123 milli-moles or 61% based on the initial amount of lysine.