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We present Very Large Telescope/FORS2 time-series spectroscopy of the Wolf–Rayet (WR) star #41 in the Sculptor group galaxy NGC 300. We confirm a physical association with NGC 300 X-1, since radial velocity variations of the He iiλ4686 line indicate an orbital period of 32.3 ± 0.2 h which agrees at the 2σ level with the X-ray period from Carpano et al. We measure a radial velocity semi-amplitude of 267 ± 8 km s−1, from which a mass function of 2.6 ± 0.3 M⊙ is obtained. A revised spectroscopic mass for the WN-type companion of 26+7−5 M⊙ yields a black hole mass of 20 ± 4 M⊙ for a preferred inclination of 60°–75°. If the WR star provides half of the measured visual continuum flux, a reduced WR (black hole) mass of 15+4−2.5 M⊙ (14.5+3−2.5 M⊙) would be inferred. As such, #41/NGC 300 X-1 represents only the second extragalactic WR plus black hole binary system, after IC 10 X-1. In addition, the compact object responsible for NGC 300 X-1 is the second highest stellar-mass black hole known to date, exceeded only by IC 10 X-1.
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