Synthesis of Complexable Fluorescent Superparamagnetic Iron Oxide Nanoparticles (FL SPIONs) and Cell Labeling for Clinical Application

We describe a new synthesis route for fluorescent superparamagnetic iron oxide nanoparticles (FL SPIONs) and the self-assembled complex formed using FL SPION and protamine sulfate for cell labeling. This synthesis route involves the conjugation of the FDA-approved dextran-coated ferumoxides with fluorescent dextran. The novel nanostructure of FL SPION is stable in vitro and it complexes with a cationic peptide.