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The ethanolysis of sunflower oil catalyzed by an immobilized 1,3‐specific porcine pancreatic lipase in a medium composed solely of substrates was investigated. The effects of the oil/ethanol molar ratio, temperature, amount of added water, and amount of enzyme used [respectively, 1∶3, 45°C, 0% (vol/vol), and 0.5 g of lipase, i.e., 10% w/w of total substrate]. To investigate the reusability of the lipase, the four‐step ethanolysis process was repeated by transferring the immobilized lipase to a substrate mixture. As a result, the percentage of conversion after the first usage decreased markedly.
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Yeşim Yeşiloğlu (2004) studied this question.
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