Key result
Transfection of the pSec Tag2A-VP2 construct in COS-7 cells resulted in the functional expression and secretion of native VP2.
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Summary VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was cloned in a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leader sequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. The construct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of VP2 was assessed by dot blotting and antigen capture ELISA. The antibody used in the immunological assays was a neutralizing monoclonal antibody (1A6) against VP2. Positive reaction with the antibody indicated the construct was functional with respect to expression and secretion of a native VP2.
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The VP2 gene of infectious bursal disease virus was successfully cloned and expressed as a secreted native protein in eukaryotic COS-7 cells.
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Ghafari et al. (2010) studied Infectious bursal disease virus. pSec Tag2A-VP2 construct was evaluated on Expression and secretion of VP2. Transfection of the pSec Tag2A-VP2 construct in COS-7 cells resulted in the functional expression and secretion of native VP2.
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