An alternative electrochemical method for the determination of proteolytic activity based on cyclic voltammetry of the electroactive probe ferricyanide (Fe(CN)63−) on the gelatin film coated ITO electrodes was proposed. The gelatin film behaves as a kinetic barrier for Fe(CN)63− and the proteolytic digestion of the gelatin film results an increase in the penetration of the Fe(CN)63−. Thus, by the change of peak potential in cyclic voltammogram of Fe(CN)63−, activity of the enzyme was determined.
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Baş et al. (2009) studied this question.
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