Hints and precautions for the care, feeding and breeding of Neurospora.

Hints and precautions for the care, feeding and breeding of Neurospora Some of these notes describe what may be established routines in older laboratories, but are either unpublished or published in inaccessible places or scattered through the old literature, and thus may not be known to those just beginning to use Neurospora.Others maybe practices unique to our lab.The attempt here is to supplement compilations of methods such as Davis and de Serres (1970 Methods in Enzymology 17A:79-143) and the older Stanford Neurospora Methods (1963 Neurospora Newsletter 4:21-25).Hints and precautions regarding particular mutants or categories of mutants will also be found in individual entries in Perkins et al. 1982 Microbiol.Rev. 46:426-570 (referred to below as "Compendium").Statements apply to N. crassa unless another species is indicated.Neurospora Newsletter is abbreviated N.N.All temperatures are °C.