A transmission electron microscopy (TEM) grid filled with 4-cyano-4′-pentylbiphenyl (5CB) on an octadecyltrichloro silane-coated glass substrate in aqueous media was developed to construct a urea biosensor by coating poly(acrylicacid- b -4-cyanobiphenyl-4-oxyundecylacrylate) (PAA- b -LCP) at the aqueous/5CB interface and immobilizing urease covalently to the PAA chains.
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Khan et al. (2014) studied this question.
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