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March 1, 1995Journal of Clinical MicrobiologyOpen Access

Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA

R. Allen Philip E. Pellett John A. Stewart Marion Koopmans

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Abstract

We developed a rapid, sensitive, and specific PCR-based assay for human cytomegalovirus (HCMV). The assay includes primer and probe sequences derived from conserved HCMV nucleotide sequences and nonradioactive hybridization-confirmation. The assay detected between 10 and 100 viral genomes. All HCMV clinical isolates tested (39 of 39) gave positive reactions.

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Allen et al. (1995) studied this question.

synapsesocial.com/papers/6a9ddb87bac4aad6ba100ec4 https://doi.org/10.1128/jcm.33.3.725-728.1995

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