INTERACTION BETWEEN Clq, Clr and Cls FROM HUMAN SERUM

The presence of Ca++ changed the electrophoretic mobility of CIs. Incubation of purified preparations of Clr and CIs in the presence of Ca++ resulted in the formation of a Clr–CIs complex, which by crossed immunoelectrophoresis was demonstrated in the β‐region. In mixtures of purified Clq, Clr and CIs containing Ca++ a complex consisting of Clq, r, s was obtained, which crossed immunoelectrophoresis demonstrated in the γ‐region. No complex formation was found between Clq and CIs in the absence of Clr.