Microstructured TiO2 films with hemispheric pores have been used here to control the size of protoplasts of the marine alga Bryopsis plumosa. The Figure shows protoplasts prepared on a 20 μm pitch TiO2 template, illustrating the small range of diameters that are obtained. This technique serves as a convenient way to provide cell models for in vitro physiological studies.
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Tatsuma et al. (2000) studied this question.