Positively Selected Sites at HCMV gB Furin Processing Region and Their Effects in Cleavage Efficiency

Figures

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  FIGURE 1. Multiple sequence alignment of the 35 unique sequences viewed by amino acids. gB1 to gB4 sequences are grouped (brackets). gB 5, 6, and 7 (dots). gBext_35 corresponds to a Gorilla gorilla cytomegalovirus gB sequence, used as external group to root the phylogenetic tree. Arrow indicates the furin cleavage site. Colors represent amino acids properties as shown in the legend.

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  FIGURE 2. Phylogenetic tree containing the 35 unique sequences. Sequences corresponding genotypes gB1 to gB4 are in different colors as indicated. The blue group contains most of the gB3 and gB6; green group contains all the gB2; red group contains all the gB1 and the gB7. Orange group contains all the gB4. The sequences containing positive selective mutations are pointed by arrows and numbered with the codon (in the alignment) which presented the mutation. The bar indicates the phylogenetic distances in terms of genetic change (bar distance = 0.06). Numbers in each node represent genetic change among branches and are expressed as the number of changes divided by the length of the sequence.

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  FIGURE 3. Furin cleavage site and sites under positive selective pressure. Red residues are positive polar, pink are negative polar, and green are neutral polar residues.

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  FIGURE 4. Bayesian Inference by MrBayes software. The probability of positive selective pressure (y-axis) plot for each site (x-axis). The cut-off of probability was 85%.