A simple and efficient HPLC analysis method has been developed to simultaneously analyse complicated multi-vitamin preparations, including vitamin B complexes, vitamin C, orotic acid, methionine, calcium pantothenate, nicotinamide, biotin, folic acid and rutin, without using expensive ion pairing reagent. Diode array detector and adjustment of the mobile phase to pH 5.6 are required in the study. This method has been validated with good linearity, reproducibility, recovery and accuracy for analysis of commercial multi-vitamin products.
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Chu et al. (1998) studied this question.
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