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Eight hundred Merino ewes in which oestrus was synchronized with progestagen.impregnated sponges were incorporated into an experiment of parallelogram design (4 X 5; n = 40) in which four doses (numbers) of spermatozoa were inseminated in five concentrations (numbers/ml), which involved a range in volume of inseminate from 0·012 ml to 1 ·6 m!. The purpose was to determine the importance on fertility of the number of spermatozoa relative to the degree of dilution, i.e. their concentration and the consequent volume in which a given number was inseminated. All ewes were inseminated 48 hr after withdrawal of sponges.
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Allison et al. (1971) studied this question.
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