An immunoelectrochemical assay in combination with homogeneous enzyme-labeled antibody conjugation was developed for rapid detection of Salmonella. The assay was performed by mixing alkaline phosphatase linked anti-Salmonella (APLAS) with Salmonella in a solution. The Salmonella-APLAS conjugate separated by polycarbonate membrane filtration was incubated with a phenyl phosphate substrate to produce phenol. The concentration of Salmonella cells was determined by measuring phenol oxidation peak current using differential pulse voltammetry at a renewable carbon paste electrode. This assay could be completed within two hours with a detection limit of 5 × 103 cells/mL. A linear response was found for Salmonella between 5 × 103 and 1 × 106 cells/mL.
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Yang et al. (1998) studied this question.