Answers are grounded in this paper and cite their sources.
An investigation was made of hydroxysteroid dehydrogenase activity in homogenates of rabbit ovaries from which the corpora lutea, if present, had been removed. Activity was determined by measuring the increase in optical density with time due to the formation of reduced pyridine nucleotide in incubation media saturated with oxidized pyridine nucleotide and hydroxysteroid. Activity was found toward steroids with hydroxyl groups in the 3β, 17β, 20α and 20β configurations. Both 3β- and 17β-hydroxysteroid dehydrogenase activities were stable for several days at either 3–5 or –20 C and were shown to.increase from pH 8.6 to 10.0. The 3β-hydroxysteroid dehydrogenase utilized DPN+ preferentially and was localized in the microsomal fraction, whereas the 17β-, 20α- and 20β-hydroxysteroid dehydrogenases preferred TPN+ and were found in the soluble fraction of an ovarian homogenate. The 3β-hydroxysteroid dehydrogenase activity (mμ/mole/ min/mg wet tissue) was found to be directly related to the weight of the ovary minus its corpora lutea, and so presumably to the amount of interstitial tissue present. Relative activities found with various steroids are reported. (Endocrinology78: 672, 1966)
No takes yet. Share an insight, caveat, or question.
Davenport et al. (1966) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: