Key result
In bovine adrenal zona glomerulosa cells, Angiotensin II increased intracellular pH and stimulated aldosterone secretion, effects that were inhibited by Na+/H+ antiport inhibitors.
Intracellular pH in bovine adrenal zona glomerulosa cells is regulated by the Na+/H+ exchange system, and Angiotensin II-induced pH changes are associated with the regulation of aldosterone steroidogenesis.
No immediate clinical implications for aldosterone regulation; leaves open the role of Na+/H+ exchange in human steroidogenesis.
Recent studies on the regulation of aldosterone biosynthesis have revealed that inhibitors of sodium influx, e.g. amiloride, can inhibit adrenal steroidogenesis with a pharmacological profile suggestive of a Na+/H+ antiport system. We have examined the existence of a Na+/H+ antiport system and its regulation of Na influx and intracellular pH (pHi) in bovine adrenal zona glomerulosa cells. NH4Cl-induced 22Na uptake by zona glomerulosa cells was dose dependently inhibited by ethylisopropylamiloride (EIPA), amiloride, and benzamil with ED50 values of 0.02, 4.30, and 199 microM, respectively. Angiotensin II (AII; 100 nM) caused an initial transient acidification, followed by prolonged alkalinization. The hormone equipotently increased pHi and stimulated aldosterone secretion, with ED50 values of 1.2 and 1.4 nM, respectively. AII-induced alkalinization was suppressed by EIPA, amiloride, and benzamil, with ED50 values of 0.6, 79, and 440 microM, respectively. This increase in pHi induced by AII was dependent upon the extracellular sodium concentration (ED50 values = 2.8 mM) and was blunted in sodium-free medium. AII-stimulated aldosterone synthesis was also inhibited by EIPA, amiloride, and benzamil, with ED50 values of 0.07, 34, and 330 microM, respectively. The time course of activation by angiotensin II on aldosterone secretion was also dependent upon extracellular sodium concentration during a 2-h period. These results document that intracellular pH is regulated through the Na+/H+ exchange system and suggest that the pH change induced by AII might be associated with its regulation of steroidogenesis in bovine adrenal zona glomerulosa cells.
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Horiuchi et al. (1989) studied Bovine adrenal zona glomerulosa cells. Angiotensin II and Na+/H+ antiport inhibitors (EIPA, amiloride, benzamil) was evaluated on Intracellular pH and aldosterone secretion. In bovine adrenal zona glomerulosa cells, Angiotensin II increased intracellular pH and stimulated aldosterone secretion, effects that were inhibited by Na+/H+ antiport inhibitors.
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