Mutation of the Blue-Green Alga, Anacystis nidulans

Cells of Anacystis nidulans in the logarithmic phase of growth were briefly treated with the mutagen, N-methyl-N'-nitro-N-nitrosoguanidine, and plated under conditions suitable for single-cell growth. Selection of aberrant colonies and examination of the cultural characteristics of these clones suggest that mutants of at least several types are easily derivable in Anacystis.