Primary cultures of adult mouse lung, spleen, and liver and of mouse embryo support the multiplication of the lactic de-hydrogenase-elevating virus. Such cultures produced virus continuously until they had been subcultured 2-3 times. This corresponded to 20 weeks in the case of lung and spleen and to 2-3 weeks with cultures of embryo. Viral multiplication was not accompanied by cytologic alterations in the cells or by changes in their rate of synthesis of nucleic acids or protein. Infection did not cause detectable changes in either the production of LDH or in its release from cells.
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Plagemann et al. (1966) studied this question.