Spectroscopic analysis reveals two-photon excited Soret fluorescence in hemoglobin, suggesting a novel approach for in vivo label-free imaging of blood vessels.
We discovered that hemoglobin emits high energy Soret fluorescence when two-photon excited by the visible femtosecond light sources. The unique spectral and temporal characteristics of hemoglobin fluorescence were measured by using a time-resolved spectroscopic detection system. The high energy Soret fluorescence of hemoglobin shows the spectral peak at 438 nm with extremely short lifetime. This discovery enables two-photon excitation fluorescence microscopy to become a potentially powerful tool for in vivo label-free imaging of blood cells and vessels.
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Zheng et al. (2010) studied this question.
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