Key result
Recombinant human periostin impregnated onto absorbable collagen sponges significantly increased osteoblast adhesion compared to unmodified sponges on day 7 (MTT absorbance 0.15 vs 0.10, p=0.002).
Why the study?
The study was conducted to evaluate the ability of periostin impregnated onto varied collagen matrices to influence osteoblast cell adhesion, proliferation, and activity.
Does recombinant human periostin impregnated onto collagen matrices improve osteoblast cell adhesion, proliferation, and activity in Saos-2 osteoblast cells?
Does recombinant human periostin impregnated onto collagen matrices improve osteoblast cell adhesion, proliferation, and activity in Saos-2 osteoblast cells?
Periostin impregnated into absorbable collagen sponges significantly increases cell viability, proliferation, and adhesion of osteoblast-like Saos-2 cells in vitro.
Does not support clinical use in periodontal regeneration; leaves open periostin-collagen matrix potential pending in vivo validation.
Purpose: The purpose of the study was to evaluate the ability of periostin when impregnated onto varied collagen matrices to influence osteoblast cell adhesion, proliferation, and activity. Materials and Methods: Saos-2 osteoblast cells were cultured and seeded onto two different collagen matrices as follows: Group A: absorbable collagen sponge (ACS), Group B: ACS impregnated with recombinant human periostin, Group C: nanocrystalline hydroxyapatite collagen (NcHC), and Group D: NcHC impregnated with recombinanant human periostin. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to evaluate cell viability as well as adhesion and proliferation on 2nd, 5th, and 7th day. Osteoblast activity was studied using alkaline phosphatase (ALP) assay for the study groups. Results: The periostin-treated absorbable collagen matrices showed a statistically significant increase in the osteoblast adhesion compared to periostin-treated NcHC on days 2, 5, and 7 (P < 0.001). The osteoblast activity as evaluated by ALP assay showed that there is increased activity in the periostin-treated ACS compared to the periostin-treated NcHC. Conclusion: From the observations of this study, it is evident that Periostin has a significant role in the modulating cellular response of the osteoblast cells. Further, incorporation of periostin into the ACS has been shown to increase the cell viability, proliferation, and adhesion of osteoblast-like Saos-2 cells.
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Subramanian et al. (2021) studied In vitro osteoblast adhesion and proliferation. Recombinant human periostin vs. Collagen matrices without periostin was evaluated on Osteoblast cell adhesion (MTT absorbance at 570 nm on day 7) (p=0.002). Recombinant human periostin impregnated onto absorbable collagen sponges significantly increased osteoblast adhesion compared to unmodified sponges on day 7 (MTT absorbance 0.15 vs 0.10, p=0.002).
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