What is the clinical evidence from this study?

Study design: Other. Intervention: Added C protein. Primary outcome: Binding of C protein to myofibrils.

What question did this study set out to answer?

To investigate the binding sites and regulatory conditions of exogenous C protein in rabbit skeletal muscle myofibrils.

July 1, 1981The Journal of Cell BiologyOpen Access

Fluorescence microscope study of the binding of added C protein to skeletal muscle myofibrils.

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Key result

Added C protein binds strongly to the I bands of rabbit skeletal muscle myofibrils in a calcium-regulated manner, and to the A band when I band binding is suppressed.

Population

Rabbit skeletal muscle myofibrils

Design

Preclinical

Authors

CMCarl MoosHeinrich Heine University Düsseldorf

Discussion

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Implication

Supports potential regulatory role for C protein in skeletal muscle; leaves open whether calcium-regulated binding occurs in human myofibrils.

Key Points

To investigate the binding sites and regulatory conditions of exogenous C protein in rabbit skeletal muscle myofibrils.
Treated rabbit skeletal muscle myofibrils with fluorescein-labeled C protein or unmodified C protein paired with fluorescein-labeled anti-C protein.
Imaged myofibrillar protein localization using fluorescence microscopy across different calcium concentrations and regional conditions.
Added C protein bound strongly to myofibrillar I bands in a calcium-regulated manner, requiring free calcium ion concentrations similar to those that activate myofibrillar ATPase.
Suppression of I-band binding revealed secondary binding in the A band, concentrated near the M line and outer edges of the thick filaments.

Structured PICO

Population

Rabbit skeletal muscle myofibrils

Intervention

Added C protein (fluorescein-labeled or unmodified plus fluorescein-labeled anti-C protein)

Outcome

Binding location and calcium regulation of C protein to myofibrilssurrogate

The binding of C protein to the I band of skeletal muscle myofibrils is calcium-regulated, suggesting potential physiological significance of C protein-actin interactions.

Cite This Study

Carl Moos (1981) studied this question. Added C protein was evaluated on Binding of C protein to myofibrils. Added C protein binds strongly to the I bands of rabbit skeletal muscle myofibrils in a calcium-regulated manner, and to the A band when I band binding is suppressed.

synapsesocial.com/papers/6a9e4912e056b05edee9871d https://doi.org/10.1083/jcb.90.1.25

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Implication

Supports potential regulatory role for C protein in skeletal muscle; leaves open whether calcium-regulated binding occurs in human myofibrils.

Fluorescence microscope study of the binding of added C protein to skeletal muscle myofibrils.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Fluorescence microscope study of the binding of added C protein to skeletal muscle myofibrils.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider