Tentoxin binding on chloroplast coupling factor 1 (CF1) was studied using a centrifugation column method followed by HPLC analysis. From non-linear regression analysis of the results, the presence of three types of binding site with the following Kd values was deduced: 6.9x10(-8) M (first site), 1.4x10(-5) M (second site), and 6.3x10(-3) M (third site). The binding of one tentoxin inhibits, that of two tentoxins moderately restores, and that of three tentoxins greatly stimulates the ATPase activity of CF1. The forward rate constant of the binding of tentoxin on the first site was 6.3x10(3) M-1 s-1.
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Mochimaru et al. (1997) studied this question.
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