Answers are grounded in this paper and cite their sources.
The influence of high pressure carbon dioxide (HPCD) on the activity and structure of pectin methylesterase (PME) from orange was investigated. The pressures were 8-30 MPa, temperature 55 degrees C and time 10 min. HPCD caused significant inactivation on PME, the lowest residual activity was about 9.3% at 30 MPa. The SDS-PAGE electrophoretic behavior of HPCD-treated PME was not altered, while changes in the secondary and tertiary structures were found. The beta-structure fraction in the secondary structure decreased and the fluorescence intensity increased as HPCD pressures were elevated. After 7-day storage at 4 degrees C, no alteration of its activity and no reversion of its beta-structure fraction were observed, while its fluorescence intensity further decreased.
No takes yet. Share an insight, caveat, or question.
Zhou et al. (2009) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: