Determination of Fumonisins B1 and B2 and Their Major Hydrolysis Products in Corn, Feed, and Meat, Using HPLC

A high-performance liquid chromatograph coupled to a fluorescence detector (excitation, 229 nm; emission, 442 nm) was used to analyze o -phthaldialdehyde-derivatized fumonisin B 1 (FB 1 ), fumonisin B 2 (FB 2 ), hydrolyzed FB 1 (HFB 1 ), hydrolyzed FB 2 (HFB 2 ), and partially hydrolyzed FB 1 (PHFB 1 ) from corn (1848 ng/g FB 1 and 1092 ng/g FB 2 ), feed (756 ng/g FB 1 and 252 ng/g FB 2 ), and spiked meat (720 ng/g FB 1, 600 ng/g FB 2, 960 ng/g HFB 1, 840 ng/g HFB 2 ) samples. Thermospray mass spectrometry was used to confirm the base hydrolysis products of FB 1 and FB 2 . Chromatography was achieved on a C 18, reversed-phase column. The mobile phases were acetonitrile/water/acetic acid mixtures set at A (40:59:1) and B (60:39:1), pumped in a gradient of 100% A to 100% B (9 min), and followed by a stepup to 100% acetonitrile (C) at the end of 17 min. The flow rate was 1 mL/min. The HPLC method has a retention factor ( k ) between 2 and 10, a selectivity coefficient (α) between 1.13 and 1.65, and a lower detection limit of 20 ng/mL at a signal to noise ratio of 5:1.